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Intended use:
The devices contained in the lipofilling kit are used to perform autologous free fat transfer. The procedure usually
involves harvesting adipose tissue with liposuction, preparing the aspirated tissue with various techniques and
injecting the prepared tissue into the receptor sites.
Target population:
For human use - Do not use on children or pregnant women
Intended users:
Health professionals only
Contraindications and side effects:
Contraindications and side effects of infiltration and sampling cannulas are described in the NUP-LT leaflet
‘HARVESTING AND INJECTION CANNULAS FOR LIPOTRANSFER’ attached to the kit
Instructions For Use:
The collection and injection of autologous adipose tissue are carried out in 1 surgical step under general or local
anesthesia.
An anesthetic solution is injected subcutaneously at the planned insertion points of the cannulas. This injection
is carried out using the 25G needle and the 3 ml syringe.
The incision site is disinfected and a small incision is made with the 14G needle. Small incisions are made in
different places depending on the area to be sampled, but are always designed to hide the surgical scar.
The tumescent solution (For example, Klein's tumescent solution: 250 ml of sodium chloride, 12.5 ml of 1%
lidocaine, 1.5 ml of 8.4% sodium bicarbonate, 0.25 ml of adrenaline 1: 1,000) is delivered to the sampling area
using the CI21150 infiltration cannula. Infiltration can be done directly with the syringe using the 20 ml or 60 ml
syringe provided in the kit. The volume of the syringe will be chosen by the user according to the volume of
tumescent solution to be injected. The cannula can also be connected to a pocket containing the infiltration
solution through a tap and the syringe will serve as a transfer device. The infiltration solution and the tap are
not supplied in the kit.
Leave the tumescent fluid to act for at least 15 minutes.
Take the fat sample using the CP141253HS cannula connected to the Vaclok® 30 ml syringe.
Transfer the fatty tissue into the 10 ml syringes using the 2.4 mm FLL / FLL connector.
Wait for sedimentation or centrifuge the fat sample (e.g. at 2,500 rpm for 4 minutes) after transfering the fat
sample into a syringe that is intended for centrifugation (not supplied in the kit).
Remove the syringe, taking care to keep it upright to avoid mixing it.
After centrifugation, three layers are observed: the first layer contains lipids, the second layer consists of adipose
tissue and the third layer contains blood, tissue fluid and infiltration solution.
Keep only the fatty tissue by eliminating the first layer with an absorbent material or by inversion and the third
layer by the lower part of the syringe.
Preparation of the fat alone:
Homogenize the fatty graft before injection, connect the 2.4 mm connector between the two 10 ml syringes and
transfer the fatty graft from one syringe to the other several times.
The fat graft can be transferred to 1 ml syringes via the 2.4 mm or 1.4 mm connector. If a further reduction in
particle size is required, the 1.4 mm or 1.2 mm connector can be used. The micronized fat is ready for injection.
Fat preparation for SVF (Stromal Vascular Fraction):
6
The devices contained in the lipofilling kit are used to perform autologous free fat transfer. The procedure usually
involves harvesting adipose tissue with liposuction, preparing the aspirated tissue with various techniques and
injecting the prepared tissue into the receptor sites.
Target population:
For human use - Do not use on children or pregnant women
Intended users:
Health professionals only
Contraindications and side effects:
Contraindications and side effects of infiltration and sampling cannulas are described in the NUP-LT leaflet
‘HARVESTING AND INJECTION CANNULAS FOR LIPOTRANSFER’ attached to the kit
Instructions For Use:
The collection and injection of autologous adipose tissue are carried out in 1 surgical step under general or local
anesthesia.
An anesthetic solution is injected subcutaneously at the planned insertion points of the cannulas. This injection
is carried out using the 25G needle and the 3 ml syringe.
The incision site is disinfected and a small incision is made with the 14G needle. Small incisions are made in
different places depending on the area to be sampled, but are always designed to hide the surgical scar.
The tumescent solution (For example, Klein's tumescent solution: 250 ml of sodium chloride, 12.5 ml of 1%
lidocaine, 1.5 ml of 8.4% sodium bicarbonate, 0.25 ml of adrenaline 1: 1,000) is delivered to the sampling area
using the CI21150 infiltration cannula. Infiltration can be done directly with the syringe using the 20 ml or 60 ml
syringe provided in the kit. The volume of the syringe will be chosen by the user according to the volume of
tumescent solution to be injected. The cannula can also be connected to a pocket containing the infiltration
solution through a tap and the syringe will serve as a transfer device. The infiltration solution and the tap are
not supplied in the kit.
Leave the tumescent fluid to act for at least 15 minutes.
Take the fat sample using the CP141253HS cannula connected to the Vaclok® 30 ml syringe.
Transfer the fatty tissue into the 10 ml syringes using the 2.4 mm FLL / FLL connector.
Wait for sedimentation or centrifuge the fat sample (e.g. at 2,500 rpm for 4 minutes) after transfering the fat
sample into a syringe that is intended for centrifugation (not supplied in the kit).
Remove the syringe, taking care to keep it upright to avoid mixing it.
After centrifugation, three layers are observed: the first layer contains lipids, the second layer consists of adipose
tissue and the third layer contains blood, tissue fluid and infiltration solution.
Keep only the fatty tissue by eliminating the first layer with an absorbent material or by inversion and the third
layer by the lower part of the syringe.
Preparation of the fat alone:
Homogenize the fatty graft before injection, connect the 2.4 mm connector between the two 10 ml syringes and
transfer the fatty graft from one syringe to the other several times.
The fat graft can be transferred to 1 ml syringes via the 2.4 mm or 1.4 mm connector. If a further reduction in
particle size is required, the 1.4 mm or 1.2 mm connector can be used. The micronized fat is ready for injection.
Fat preparation for SVF (Stromal Vascular Fraction):
6
